Even though she's sorta goofy looking, I always liked the Queen Bee. Super-villainesses were rare enough, but one powerful enough to take on the whole JLA?
Plus she was kinda hot. Have I said too much?
The story: "Drones of the Queen Bee!" by Gardner Fox, Mike Sekowsky, and Bernard Sachs. The Queen Bee uses her powers to force the JLA to procure elements that when combined will produce an immortality serum for her!
Roll Call: Superman, Batman, Wonder Woman, Aquaman, Flash, Green Lantern, Martian Manhunter, Green Arrow, The Atom
Notable Moments: Even though Superman, Batman, and Aquaman are listed in the Roll Call, they play no part in this story and show up only as the very end.
Also, the Atom misses the story entirely, because he was off having another adventure in his own book(appearing in The Atom #8, Julius Schwartz helpfully informs us), so the issue ends with Atom about to regale the JLA with the story. Weird!